Construction and verification of adenovirus containing the PUMA gene under the control of the hTER promoter
10.3969/j.issn.1671-8348.2013.36.026
- VernacularTitle:hTERT启动子调控的PUMA重组腺病毒的构建与鉴定
- Author:
Dong WANG
;
Qi ZHOU
;
Yucong LI
- Publication Type:Journal Article
- Keywords:
telomerase terminal transferase;
p53 up-regulate modulator of apoptosis;
genetics;
adenoviridae
- From:
Chongqing Medicine
2013;(36):4430-4432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construt the adenovirus vectors of Ad-hTERT-PUMA-EGFP containing human telomerase reverse transcriptase(hTERT) ,PUMA and enhanced green fluorescent protein (EGFP) .Methods By a series of molecular biology tech-niques ,PUMA gene was inserted into downstream of the hTERT promoter .And then EGFP was inserted into downstream of the PUMA in adenovirus vectors .Finally ,recombinant adenovirus vector ,Ad-hTERT-PUMA-EGFP was assembled into the adenovi-rus .Results Correct adenovirus laoding vectors was verified by viral genome PCR technology .After 12 h of transfection ,HEK293 cells showed green fluorescence in 72 h .Conclusion The correct structure of the adenovirus vectors containing PUMA gene and EGFP gene was identified by restriction enzyme analysis .