Effect of APE1/Ref-1 on differentiation of peripheral blood mononuclear cells into osteoclast-like cells
10.3969/j.issn.1671-8348.2013.34.003
- VernacularTitle:APE1／Ref-1在外周血单核细胞破骨样分化中的作用研究
- Author:
Jia DU
;
Jiayin XIE
;
Liang ZHANG
;
Liwei ZHOU
;
Yuxin YANG
;
Dong WANG
- Publication Type:Journal Article
- Keywords:
mononuclear cells;
osteoclast cells;
cell differentiation;
apurinic/apyrimidinic endonuclease/redox-factor1
- From:
Chongqing Medicine
2013;(34):4112-4114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of APE1 on differentiation of peripheral blood mononuclear cells into osteoclast-like cells(OCL) which induced by macrophage colony stimulating factor (RANKL) and macrophage colony stimulating factor (M-CSF) .Methods Human peripheral blood mononuclear cells (PBMCs) were collected by density gradient separation ;Constructed APE1 siRNA expression vector Ad5v-APE1 siRNA was used to transfect PBMCs .Tartrate-resistant acid phosphatase (TRAP) method was conducted to identify the cells ,the expression level of APE1 was detected by Western blot ,the mRNA expression levels of Cathepsin K(CK) and V-ATPase were detected by RT-PCR .Results PBMCs transfected with APE1 siRNA had significantly lower protein expression of APE1 than untransfected cells (P< 0 .05) ;PBMCs could differentiate into OCL under the stimulation of RANKL and M-CSF ,the mRNA expression levels of CK and V-ATPase increased ;After APE1 siRNA treatment ,the number of OCL was reduced and the levels of CK and V-ATPase mRNA decreased .Conclusion PBMCs can differentiate into a large number of OCL induced by RANKL and M-CSF ,APE1 siRNA significantly inhibited differentiation of PBMCs into osteoclast-like cells , APE1 may be involved in the regulation of osteoclast-like differentiation process .
