Preliminary study on the immunogenicity of an experimental encephalomyocarditis virus vaccine

VernacularTitle:脑心肌炎病毒实验性疫苗免疫原性初步研究
Author: Jing XU ; Shuxiang LI ; Jing LIU ; Xinyi WANG ; Ye YUE ; Yuan LING
Publication Type:Journal Article
Keywords: Encephalomyocarditis virus; Immunogenicity; Median lethal dose
From:Chinese Journal of Microbiology and Immunology 2013;(12):950-953
CountryChina
Language:Chinese
Abstract: Objective To prepare an experimental encephalomyocarditis virus ( EMCV) vaccine and study its immunogenicity .Methods EMCVs were cultured in Vero cells and viral titer was detected by micro cytopathy method .A series of procedures including concentration , filtration and ultracentrifugation were conducted to purify EMCVs .Formaldehyde and β-propiolactone were used for viral inactivation and their efficacy was evaluated .The median lethal dose ( LD50 ) of EMCV was measured by using Reed-Muench method.The serum antibody titers in BALB/c mice vaccinated with different immunization strategies were analyzed through microneutralization assay .Then the immunized mice were challenged with 100LD50 , 50LD50 and 25LD50 of EMCV by intraperitoneal injection .Results The viruses could be inactivated by β-propiolactone at a volume ratio of 1 ∶4000 for 12 hours at 4℃.The LD50 of EMCV was 17 CCID50/ml.The immunized mice produced neutralizing antibodies and displayed resistance to EMCV infection.Conclusion The experimental EMCV vaccine could induce protective antibodies in mice .