Establishment of a concentration test method of S-071031B in rat plasma and its pharmacokinetics
10.7644/j.issn.1674-9960.2013.11.004
- VernacularTitle:大鼠血浆中抗抑郁新药阿姆西汀浓度检测方法的建立及其药代动力学
- Author:
Xiuqing ZHU
;
Jinglai LI
;
Ming DENG
;
Shan XIONG
;
Zhenqing ZHANG
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
S-071031 B;
rat plasma;
pharmacokinetics
- From:
Military Medical Sciences
2013;(11):818-821
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an LC-MS/MS method for determination of S-071031 B, a novel antidepressant , in rat plasma and to study its pharmacokinetic profiles .Methods An LC-MS/MS method was established to determine S-071031B in rat plasma, and L-8021 was employed as the internal standard .The analytes were separated on a C18 column with a mobile phase consisting of water-acetonitrile containing 0.1%(v/v) formic acid at a flow rate of 0.3 ml/min.The mass spectrometer was operated in a selected reaction monitoring ( SRM ) mode with a positive electrospray ionization (ESI) interface.The plasma concentration-time curve was drawn and pharmacokinetic parameters were calculated by DAS 2.0.Results The linear range was from 2 to 1000 ng/ml with a sensitivity of 2 ng/ml as the lower limit of quantification . The intra-day and inter-day precisions , recoveries and matrix effects at three spiked levels were all suited to the determina-tion of biological samples.After oral administration of S-071031B, the Cmax of S-071031B was (287.2 ±50.8) μg/L and the Tmax was (0.8 ±0.3) h, with a t1/2of (2.9 ±0.6) h and an AUC(0-∞)of (1372.6 ±255.3) μg/L· h.Conclusion This method is sensitive and specific enough for determination of S-071031 B in rat plasma to facilitate the study of its phar-macokinetics .
