Deficiency of Erbin in MDA-453 human breast cancer cells induces trastuzumab resistance in vitro
10.7644/j.issn.1674-9960.2013.11.007
- VernacularTitle:Erbin表达缺失诱导MDA-453人乳腺癌细胞对曲妥珠单抗耐药
- Author:
Yabin HU
;
Limin SUN
;
Hongyu CHEN
;
Ming YU
;
Meiru HU
;
Ming SHI
- Publication Type:Journal Article
- Keywords:
Erbin;
breast neoplasms;
trastuzumab;
drug resistance
- From:
Military Medical Sciences
2013;(11):831-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Erbin deficiency in MDA-453 cells on trastuzumab(Herceptin) resist-ance .Methods The specific short hairpin RNA ( shRNA) targeting Erbin was designed and cloned into plasmid pSuppres-sor, which was subsequently transfected into MDA-453 human breast cancer cells .After being selected by G418, MDA-453 cells stably expressing Erbin shRNA were obtained and nominated as MDA-453-Erbin sh.The MDA-453 cells express-ing control shRNA ( MDA-453-NC) were used as control cells .The expression of Erbin at the protein level in MDA-453-NC and MDA-453-Erbin sh cells was analyzed by Western blotting .Cell proliferation and colony formation assays were em-ployed to investigate the effect of Erbin knockdown on the sensitivity of MDA -453 cells to trastuzumab in vitro.The levels of Erbin expression in human breast cancer tissue and normal breast tissue samples were evaluated by immunohistochemistry . Results MDA-453 cells, in which Erbin expression was stably knocked-down, were established.Deficiency of Erbin in MDA-453 cells could antagonize the anti-proliferation effects of trastuzumab in vitro.The level of Erbin expression was de-creased in some breast cancer tissue samples compared with normal breast tissue samples .Conclusion Erbin deficiency may induce the resistance of breast cancer cells to trastuzumab therapy .
