Methylation of E-cadherin, p16, RASSF1A, DAPK and MGMT in salivary gland tissues
10.7644/j.issn.1674-9960.2013.11.009
- VernacularTitle:正常涎腺组织中E-cadherin，p16，RASSF1 A，DAPK和MGMT基因甲基化检测
- Author:
Chunye ZHANG
;
Xuxia DENG
;
Meijuan ZHOU
;
Hongnan YU
;
Lei LI
;
Hanbing FU
;
Jiang LI
- Publication Type:Journal Article
- Keywords:
salivary gland;
DNA methylation;
E-cadherin;
genes,p16;
RASSF1A;
DAPK
- From:
Military Medical Sciences
2013;(11):839-842
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the methylation status of E-cadherin(E-cad), p16, RASSF1A, DAPK and MGMT in histologically normal salivary gland tissues and provide reference for determination of the methylation status of salivary gland tumors.Methods Methylation of E-cad, p16, RASSF1A,DAPK and MGMT was analyzed using methylation-specific polymerase chain reaction ( MSP) .The results were compared with the methylation status of these genes in salivary adenoid cystic carcinoma ( ACC) tumor tissues in our previous studies and the association between promoter methylation of E-cad, p16, RASSF1A, DAPK, and MGMT on one hand and the patients′gender, age, smoking and types of gland on the other hand was also analyzed .Results Promoter methylation was detected in 8 of the 60 (13%) salivary glands, E-cad in 4(7%), p16 in 2(4%), RASSF1A in 2(4%), DAPK in 2 (4%), and MGMT in 1(2%).Compared with our previous results, there was a significantly lower methylation ratio in promoter methylation of E-cad(P<0.01), p16 (P<0.01), RASSF1A (P<0.01),and DAPK (P<0.01) in salivary gland tissues than in ACC tumor tissues.Conclusion Promoter methylation of E-cad, p16 and RASSF1A is a rare event in histologically normal salivary gland tissues .
