Influence of Gleevec on expression of miR-146a,miR-29b and DNMT in K562 cells

VernacularTitle:格列卫对 K562细胞中miR-146a、miR-29b及3种甲基化酶表达的影响
Author: Lina WANG ; Jianming ZENG ; Huacheng WANG ; Mo LI ; Yifei LONG ; Guangyuan DENG ; Cha CHEN
Publication Type:Journal Article
Keywords: microRNA146a; chronic myelogenous leukemia(CML); DNMT; Gleevec
From: Chongqing Medicine 2014;(3):301-303
CountryChina
Language:Chinese
Abstract: Objective To investigate the changes of microRNA (miR)-146a ,miR-29b expression levels and the 3 kinds of meth-ylase DNMT1 ,DNMT3a and DNMT3b levels in K562 cell lines after BCR/ABL inhibitor Gleevec treatment .Methods The half maximal inhibitory concentration(IC50 ) of Gleevec on K562 cells was detected by the MTT method .The stem loop primers method and the fluorogenic quantitative PCR were adopted to detect miRNAs and the methylase gene level .Results IC50 of Gleevec acting on K562 cells was 40 .85μmol/L .After Gleevec action ,miR-29b showed the increasing trend ,but 3 kinds of methylase expression level were decreased to some extent .Gleevec could significantly increase the miR-146a level in K562 cells(P<0 .05) .Conclusion Gleevec can influence the expression of miR-146a ,miR-29b and DNMTs levels in K562 cells .