SRF expression and its biological significance in gastric carcinoma cells
10.3969/j.issn.1000-8179.20131423
- VernacularTitle:SRF在胃癌细胞中的表达及其意义
- Author:
Jing ZUO
;
Liang LIU
;
Min ZHAO
;
Lianfu ZUO
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
serum response factor;
flow cytometry
- From:
Chinese Journal of Clinical Oncology
2014;(3):166-169
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to explore the relationship between serum response factor (SRF) expression level and gas-tric cancer progression by detecting SRF expression level in cancer cells. Methods:The SRF gene in SGC-7901 cells was silenced by RNA interference. Transfection efficiency was detected by fluorescence microscopy, cell proliferation by CCK 8 method, SRF gene and protein expression level by real-time polymerase chain reaction and Western blot, and cell cycle by flow cytometry. Results:Cell treat-ment with siRNA-SRF induced significant reduction in SRF mRNA levels. Western blot analysis showed that SRF protein decreased by 40.1%in the siRNA group compared with that in the control group (P<0.05). Compared with the blank, negative, and mock transfection control groups, cell proliferation of the siRNA-SRF group decreased. The inhibition ratio reached 64.24%, as measured by the CCK-8 assay (P<0.05). Treatment with siRNA could block SGC-7901 cell cycle at G0/G1 phase (P<0.05). Conclusion:SRF expression is close-ly associated with gastric carcinoma cell proliferation. SRF protein level detection can provide a certain reference value in evaluating malignant gastric carcinoma progression. SRF is possibly an important target for the prevention and control of gastric cancers.
