To investigate the immunity characteristics of a conserved sequence on the C terminal region of the human papillomavirus major protein L 1
10.3969/j.issn.1671-8348.2014.01.004
- VernacularTitle:HPV L1 C-末端保守序列多肽免疫学特性的探讨
- Author:
Xuemei JIANG
;
Changyi XIAO
;
Jiangfeng WU
;
Hongjun LI
;
Taining YUAN
;
Guicehng TANG
- Publication Type:Journal Article
- Keywords:
HPV;
late protein;
conserved sequence;
immunogenicity
- From:
Chongqing Medicine
2014;(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and detect the immunity characteristics of a conserved sequence including 30 amino residues which is located in the C terminal of HPVL1 .Methods The immune model of mouse was establish with a polypeptide synthetized based on this sequence .The amount of CD3+ ,CD3+CD4+ or CD3+CD8+ lymphocyte of the mouse spleen was detected by Flow cy-tometry ,then the value of CD4+ /CD8+ were calculated .The cell proliferation was detected by MTT assay .Sample was took from the cell supernatant to detect the content of IL-4 and IFN-γby double antibody sandwich ELISA .Results (1)The amount of CD3+CD8+ lymphocytes and the value of CD4+ /CD8+ were significantly increased than control group(P<0 .05) .(2)The result of T cell proliferation showed no significant difference (P>0 .05) .(3)The level of IL 4 were significantly higher than that of control group (P<0 .01) .The content of IFN-γlevel showed no significant difference compared with control group (P>0 .05) .Conclusion The results showed that it was affirmative that the polypeptides induced humoral immunity ,which was worth to be furtherly studied as a preventive vaccine .But its cell immunogenicity is weak ,and the attenmpt to induce the response related to the cell immunogenicity was failed ,and the immunogenicity of the peptide remains to be improved .
