Evaluation of acute liver injury in mice model with different does of CCl 4
10.3969/j.issn.1671-8348.2014.01.006
- VernacularTitle:不同剂量CCl4急性肝损伤模型的评价
- Author:
Daoming LIANG
;
Zhixing HU
;
Min LUO
;
Yi ZHANG
;
Jiayong CHEN
- Publication Type:Journal Article
- Keywords:
hepatocyte transplantation;
acute liver injury;
carbon tetrachloride
- From:
Chongqing Medicine
2014;(1):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple ,stable acute liver injury model induced by CCl4 to observe effects of hepatocyte transplantation .Methods CCl4 plant oil with different concentration of 20% and 50% was used in mice by intraperitoneal injection , of which the dose was 2 mL/kg ,and then materials were taken at different time points respectively .Mice survival rate ,alanine amin-otransferase (ALT) ,aspartate aminotransferase (AST) and the pathological changes of the liver were detected .Results Mice sur-vival rate in 20% CCl4 intraperitoneal injection was significantly higher than that of 50% .ALT and AST in experiment group were significantly higher than that of control group ,but there was no significant difference between two experimental groups .Pathologi-cal examination showed that mice liver cells showed typical cytoplasmic ,ballooning ,scattered punctate ,piecemeal necrosis and in-flammatory cell infiltration in 20% CCl4 intraperitoneal injection ;while in 50% CCl4 ,there was obvious fibrosis ,in addition to the mentioned heavier lesions .Conclusion 20% -50% CCl4 intraperitoneal injection in 2 mL/kg dose can induce different degrees of relatively stable liver injury ,and its concentration determines the degree of liver injury .Acute liver injury induced by 20% -50%CCl4 was an ideal model for hepatocyte transplantation experiment .
