Experimental Study on Antitumor Effect of Volatile Oil of Xihuang Pill and Its Immune Mechanism
10.11842/wst.2014.01.012
- VernacularTitle:西黄丸挥发油抗肿瘤作用及其免疫学机制的实验研究
- Author:
Wei YANG
;
Shuo GUAN
;
Junxia HU
;
Changqian ZENG
;
Wenbo LIANG
;
Jie MA
;
Wenbin GAO
- Publication Type:Journal Article
- Keywords:
Volatile oil of Xihuang pill;
walker 256 breast cancer cell;
rat;
cytokine;
T-cell subset;
B7-1 cell;
immune clearance
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the antitumor effect of volatile oil of X ihuang pill and its immune mecha-nism in order to screen the antitumor active site of Xihuang pill. Among 70 female Wistar rats, 10 rats were random-ly selected as the blank control group; and the other 60 rats was used in the establishment of walker 256 breast can-cer cell tumor-bearing rat models. The model rats were randomly divided into the negative control group (model group), high-dose volatile oil group, middle-dose volatile oil group, low-dose volatile oil group, high-dose Xihuang pill group, and lentinan group (positive control group), with 10 rats in each group. The intragastric administration was given twice a day for 14 days. Blood was taken from the abdominal aorta. Tumor tissues was removed and weighed to calculate the tumor inhibitory rate. ELISA method was used to detect the level of IL-2, IL-6, IL-10, IFN-γ and TGF-β in peripheral blood. The flow cytometry was used to detect the content of CD3+ T cell, CD4+ T cell, CD8+ T cell, and B7-1 cell (CD80). The results showed that the tumor inhibitory rates of volatile oil of high-dose group and middle-dose group were 28.4% and 24.1%, respectively. Compared with the model group, the average level of IL-2 and IFN-γ of volatile oil of high-dose group and middle-dose group and CD3+ T cell, CD8+ T cell, B7-1 cell con-tent were obviously increased (P< 0.05). It was concluded that volatile oil of Xihuang pill had certain antitumor ef-fect, which was one of the antitumor active sites of Xihuang pill. The volatile oil of Xihuang pill upregulates the lev-els of IL-2 and IFN-γ, as well as the contents of CD3+ T cell, CD8+ T cell, B7-1 cell in order to increase the im-mune clearance function of tumor-bearing rats.
