Effect of Xiao-Chai-Hu Decoction with Different Doses of Bupleurum on Body Temperature and Content of IL-1β, IL-6, TNF-α in Serum Induced by LPS among Fever Rat Models
10.11842/wst.2014.01.010
- VernacularTitle:不同柴胡剂量小柴胡汤对LPS诱导发热大鼠模型体温及血清IL-1β、IL-6、TNF-α的影响
- Author:
Feng CAO
;
Amei TANG
- Publication Type:Journal Article
- Keywords:
Dose of Bupleurum;
Xiao-Chai-Hu Decoction;
lipopolysaccharide;
antifebrile
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(1):58-62
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the effect of Xiao-Chai-Hu Decoction (XCHD) with different doses of Bupleurum on body temperature and content of IL-1β, IL-6 and TNF-α in serum among fever rat models induced by lipopolysaccharide (LPS) in order to and investigate its action mechanism. A total of 50 rats were randomly divid-ed into 5 groups, including the normal control group, fever model group, XCHD group (high-dose), XCHD group (middle-dose), and XCHD group (low-dose). The fever rat model was established by intraperitoneal injection of LPS (20 μg/kg). At the same time, all rats were given corresponding XCHD or saline solution by gavage. The body tem-perature of rats (Ti) was measured every 30 min for 240 min after the injection of LPS. Change values of body tem-perature △T(△T=Ti-T0) were calculated. After the last measurement of body temperature, blood was taken for the de-tection of IL-1β, IL-6 and TNF-α level in serum with ELISA. The results showed that the body temperature of XCHD group (high-dose) was obviously decreased at the time point of 3 h, 3.5 h, 4 h (P< 0.01, or P< 0.05). This temperature reducing effect at the time point of 3.5 h and 4 h was better than the XCHD group (middle-dose) and XCHD group (low-dose, P< 0.01, or P< 0.05). Compared with the fever model group, all three XCHD groups can obviously reduce the IL-1β level (P< 0.05). Among them, the XCHD group (high-dose) had obvious effect (P<0.01). Compared with the fever model group, the XCHD group (high-dose) can obviously reduce contents of IL-6 and TNF-α in serum (P< 0.05). It was concluded that XCHD can reduce fever induced by LPS in rats and decrease contents of IL-1β, IL-6 and TNF-α in serum. This effect has a positive dose-effect relationship with the dose of Bu-pleurum in a certain range.
