Regulation of Insulin Signaling in the Hypothalamus of Spleen Yin Deficiency Diabetes Rats Treated with Zibu Piyin Recipe
10.11842/wst.2014.01.015
- VernacularTitle:滋补脾阴方药对脾阴虚糖尿病大鼠下丘脑胰岛素信号的调控作用
- Author:
Lina LIANG
;
Libin ZHAN
;
Luping ZHENG
;
Shouyu HU
;
Yun YAN
;
Hua SUI
;
Fuliang ZHANG
- Publication Type:Journal Article
- Keywords:
Spleen yin deficiency diabetes;
cognitive decline;
brain insulin resistance;
Zibu Piyin Recipe
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(1):82-86
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe changes of key molecular in insulin signaling pathway in the hypothala-mus of rats to explore the mechanism of spleen yin deficiency diabetes-associated cognitive decline (DACD) and Zibu Piyin Recipe (ZBPYR) in order to provide new ideas and new clues for treatment of DACD. Rats were randomly divided into five groups, which were the control (Cont) group, diabetes (DM) group, spleen yin deficiency (pi) group, spleen yin deficiency diabetes (piDM) group and the ZBPYR group. Insulin receptor substrate 1 (IRS-1) serine phos-phorylation levels and protein kinase B (PKB/Akt) serine phosphorylation levels which were involved in the insulin signaling were observed by western blotting in the hypothalamus to determine whether there were insulin signaling obstacles in the hypothalamus of rats. The results showed that the expression of p-IRS-1ser in the DM group, pi group and piDM group was increased compared with the Cont group (P< 0.05); while the p-Akt expression of the DM group and piDM group was decreased (P< 0.05). The expression of p-IRS-1ser in the ZBPYR group decreased compared with the DM group and piDM group (P< 0.05); while the level of p-Akt increased compared with the DM group and piDM group (P < 0.05). It was concluded that insulin signaling was not transduced normally in the hy-pothalamus of the DM group, pi group and piDM group. Insulin resistance may occur in the hypothalamus. And ZBPYR can correct insulin signaling transduction disorder.
