Simultaneous Determination of Five Active Chemical Components in Tibetan Medicine Pterocephalus Hookeri Hoeck by UFLC-PDA
10.11842/wst.2014.01.030
- VernacularTitle:UFLC-PDA同时快速测定藏药翼首草中5种化学成分的含量
- Author:
Wenjie LI
;
Yan GAO
;
Yilong CHEN
;
Yujie WANG
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
Tibetan medicine;
P. hookeri;
chlorogenic acid;
loganin;
sweroside;
evodia rutaecarpa glycosides;
triplostoside A;
UFLC-PDA
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(1):161-166
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an Ultra Fast Liquid Chromatography-Photo Diode Array (UFLC-PDA) method for the simultaneous determination of five chemical components, which included chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, in Pterocephalus hookeri h eck. Agilent Poroshell 120 SB-C18 (100 mm í 4.6 mm, 2.7 μm) was adopted, with acetonitrile-0.2% phosphoric acid solution in gradient elution as the mobile phase at the flow rate of 1.0 mL·min-1. And the injection volume was 0.4 μL. The detection wavelength was set up at 237 nm and 325 nm. And the column temperature was 30℃. The results showed that the calibration curve was linear within the range of 8.72~218.0, 1.52~38.0, 2.44~61.0, 29.36~734.0, 3.00~75.0μg·mL-1 (r > 0.999 6, n=9) for chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, respectively. The average recovery rates were 99.46%, 99.41%, 100.14%, 98.89%, and 99.42%, respectively. The RSD was 0.69%, 0.66%, 0.60%, 1.21%, and 0.64%, respectively (n = 9). It was concluded that this method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of five chemical components in P. hookeri.
