Optimization of Alcohol Precipitation Techniques for Honeysuckle Extract of Qingkailing Injection by Orthogonal Test
10.11842/wst.2014.01.035
- VernacularTitle:正交试验法优化清开灵注射液中金银花提取液的醇沉工艺
- Author:
Xiuyu SUN
;
Yingzi WANG
;
Yanjiang QIAO
;
Ailing FENG
;
Feipeng DUAN
;
Haiyan ZHOU
- Publication Type:Journal Article
- Keywords:
Qingkailing Injection;
honeysuckle;
orthogonal test;
alcohol precipitation;
chlorogenic acid;
galuteolin
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(1):187-192
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to optimize the alcohol precipitation techniques for honeysuckle extract in order to standardize the production of honeysuckle extract of Qingkailing Injection and reduce the differences among batches. The orthogonal test was applied in this study. The content of chlorogenic acid and galuteolin were taken as compre-hensive indicators. Multi-index comprehensive scoring method was used in the data analysis. Three influencing fac-tors, which were the fluid temperature, the stirring speed and the speed of adding alcohol, were optimized in the al-cohol precipitation techniques. The results showed that the optimal alcohol precipitation techniques were when the fluid temperature was 20℃, the stirring speed was 240 rpm and the speed of adding alcohol was one time of the ma-terial per minute. It was concluded that the optimized alcohol precipitation process was stable and feasible.
