Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens.
- Author:
Kwi Sung PARK
1
;
Kyoung Ah BAEK
;
Dong Uk KIM
;
Kyung Sook KWON
;
Sun Hye BING
;
Joon Soo PARK
;
Hae Seon NAM
;
Sang Han LEE
;
Young Jin CHOI
Author Information
- Publication Type:Brief Communication ; Comparative Study ; Evaluation Studies
- Keywords: Immunochromatographic assay; Norovirus; Sensitivity; Specificity
- MeSH: Acute Disease; Adolescent; Adult; Aged; Aged, 80 and over; Caliciviridae Infections/*diagnosis; Child; Child, Preschool; Feces/*virology; Gastroenteritis/*diagnosis/virology; Humans; *Immunoassay; Infant; Middle Aged; Norovirus/*genetics/isolation & purification; RNA, Viral/analysis; Reagent Kits, Diagnostic; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity
- From:Annals of Laboratory Medicine 2012;32(1):79-81
- CountryRepublic of Korea
- Language:English
- Abstract: Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
