Survivin mRNA is suppressed in HepG2 cells after AFP gene silencing

VernacularTitle:沉默AFP基因可抑制肝癌HepG2细胞Survivin mRNA的表达
Author: Nian FANG ; Ziling FANG ; Huiqing ZHANG ; Xiaoni HAN ; Gen HUANG ; Nongrong WANG ; Jianping XIONG ; Kunhe ZHANG
Publication Type:Journal Article
Keywords: hepatocellular carcinoma; AFP; Survivin; RNA interference
From: Chinese Journal of Clinical Oncology 2013;(24):1528-1530
CountryChina
Language:Chinese
Abstract: Objective:To observe the effects of AFP gene silencing by siRNA on the Survivin mRNA of hepatocellular carcino-ma cell line HepG2. Methods:AFP gene expression was downregulated in HepG2 cell by RNAi, and the AFP content in the superna-tant was detected by ELISA. Survivin mRNA level was tested by RT-PCR. MTT was applied to evaluate cell proliferation. Flow cytom-etry was employed to observe cell apoptosis. Results:At 48 h after transfection, AFP expression was almost completely inhibited, cell proliferation activity was decreased by 43.1%, cell apoptosis rate was increased by 24.3%, and the Survivin mRNA expression was re-duced to 22.0%in the experimental group. No evident changes were observed in negative control and blank groups. Conclusion:AFP gene silenced by RNAi induces growth inhibition and apoptosis promotion of hepatocellular carcinoma cell line HepG2. This gene may be associated with the suppression of Survivin mRNA.