Analysis on gallic acid in paeonia suffruticosa andr. by HPLC-MS
10.3969/J.ISSN.1672-8270.2013.12.003
- VernacularTitle:液相色谱-质谱联用法测定牡丹皮中没食子酸的含量
- Author:
Guang ZHAO
;
Jun LI
;
Xuejing DU
- Publication Type:Journal Article
- Keywords:
Paeonia suffruticosa Andr;
HPLC-MS;
Gallic acid;
Fatty acid synthase
- From:
China Medical Equipment
2013;(12):8-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect gallic acid in Paeonia suffruticosa Andr. by high performance liquid chromatography-mass spectrometry. Methods: A method for determining gallic acid in Paeonia suffruticosa Andr. by HPLC-MS is described. The operating conditions were Diamonsil C18 column (5μm, 4.6×250 mm) at room temperature, methanol-0.05% phosphoric acid solution as mobile phase by step gradients elution at a flow of 0.8 ml/min and UV detection at 270 nm. Results:The content of gallic acid is 1.8 mg/g. The relative standard deviation of gallic acid is 1.7%. Conclusion:The content of gallic acid in Paeonia suffruticosa Andr. is high, which may be related to the inhibition of FAS.
