Content Determination of Three Ginsenosides in Shengmai Ultra-micro Powder

VernacularTitle:生脉超微粉中3种人参皂苷含量测定方法学研究
Author: Xinjian QIU ; Shouxin LI ; Wuzhan LIU ; Ruiqiang SU ; Zeping ZHANG ; Zhiquan ZHAO
Publication Type:Journal Article
Keywords: HPLC; Shengmai ultra-micro powder; ginsenoside Rg1; ginsenoside Re; ginsen oside Rb1; content determination
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(8):1801-1804
CountryChina
Language:Chinese
Abstract: This study was aimed to establish an HPLC method to determine three ginsenosides in Shengmai ultra-micro powder. The kromasil C18 (250 mm í 4.6 mm, 5 μm) was used as analytical column. The mobile phase was composed of acetonitrile (A) and water (B) with gradient elution (0~35 min, 19% A; 35~55 min, 19%~29% A; 55~70 min, 29% A; 70~100 min, 29%~40% A) at a flow rate of 1 mL·min-1. The detection wavelength was 203 nm and the column temperature was 30℃. The injection volume was 10 μL. The results showed that the linear ranges of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 were 0.083~0.834 μg, 0.086~0.863 μg, 0.091~0.911 μg, respec-tively. The average recovery rates (n = 6) were 100.7%, 100.5%, 100.5%, respectively. It was concluded that this method was quick, sensitive, repeatable and suitable to determine contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Shengmai ultra-micro powder.