The radiosensitizing effects of cryptotanshinone on H22 hepatoma-bearing mice
10.3969/j.issn.1007-3969.2014.01.005
- VernacularTitle:隐丹参酮对肝癌H22荷瘤小鼠放射增敏作用的影响
- Author:
Yintao YE
;
Chen WANG
;
Xiaokun SONG
;
Jie ZHANG
;
Ning GAO
- Publication Type:Journal Article
- Keywords:
Cryptotanshinone;
H22 hepatoma;
Radiosensitization;
Cell cycle;
Apoptosis
- From:
China Oncology
2014;(1):29-34
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Quinonoids can change the cell cycle distribution of tumor cells, and effect the radiosensitizing. This study aimed to investigate the radiosensitization effects, cell cycle and apoptosis of cryptotanshinone on H22 hepatoma-bearing mice. Methods:The mouse hepatoma H22 model was established, then divided into blank control group, irradiation alone group, high dose of cryptotanshinone group, cryptotanshinone (low, medium and high)+IR groups. After irradiated, observed the growth of tumor’s conditions, record epigenetic tumor irradiation time, calculated the delay time of tumor growth and enhancement factor (EF). After 22 days, mice were killed, stripped tumor, and calculated the inhibition rate. The cell cycle distribution and apoptosis were measured by lfow cytometry. Results:Cryptotanshinone (low, medium and high) groups inhibited the tumor growth better than the blank group, and had the signiifcant radiosensitizing effect. The enhancement factor was 1.22, 1.43,2.19, respectively. Cells were treated with cryptotanshinone which had significant effects on cell cycle, and induced apoptosis, which indicated signiifcant G2/M phase arrest and a decrease in S phase. Conclusion:Cryptotanshinone inhibited the tumor growth and had the radiosensitizing effects on H22 hepatoma-bearing mice. One of the mechanism may be that it might make significant G2/M phase arrest and S phase decreased, and induced apoptosis. So cells were more sensitive to radiation.
