Construction and identification of pcDNA3-HBsAg-p30-ROP2 expression vec-tor
- VernacularTitle:pcDNA3-HBsAg-p30-ROP2真核表达载体的构建与鉴定
- Author:
Qingkuan WEI
;
Yingting WANG
;
Yunqin YAN
;
Ting XIAO
;
Jin LI
;
Chao XU
;
Gongzhen LIU
;
Meijuan LIU
;
Weixia ZHONG
;
Kun YIN
;
Bin FU
;
Ge YAN
;
Bingcheng HUANG
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Surface antigen 1(p30);
Rhoptry protein 2(ROP2);
HBsAg;
Gene recombination
- From:
Chinese Journal of Schistosomiasis Control
2014;(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a multi-gene recombinant pcDNA3-HBsAg-p30-ROP2 expression vector and identify it preliminarily. Methods According to recombinant pcDNA3-p30-ROP2 restriction sites,HBV HBsAg gene sequences of primers were designed and synthesized to amplify target fragment,and then cloned into pcDNA3-HbsAg-p30-ROP2 expression vector. Af-ter sequencing,it was identified finally by restriction enzyme digestion and other molecular biology techniques. Results HBV HBsAg gene segment was amplified by PCR and the multi-gene recombinant pcDNA3-HBsAg-p30-ROP2 expression vector was constructed and identified to be correct as theoretical values. The PCR and restriction enzyme digestion results showed that HBsAg and p30-ROP2 gene in recombinant plasmid were confirmed by DNA sequencing. Conclusion The multi-gene recombinant pcD-NA3-HBsAg-p30-ROP2 expression vector is successfully constructed.
