Expression of CNP/NPR-B axis and NPR-C/NEP elimination pathways in rats with unilateral ureteral obstruction

VernacularTitle:CNP/NPR-B效应轴与NPR-C/NEP清除途径在大鼠梗阻肾脏的表达
Author: Xueqi ZHAO ; Jing WANG ; Haibo KONG ; Bo HU ; Peng HU
Publication Type:Journal Article
Keywords: C-type natriuretic peptide; natriuretic peptide receptor; neutral endopeptidase; tubulointerstitial fibro-sis; unilateral ureteral obstruction
From: Journal of Clinical Pediatrics 2014;(2):170-177
CountryChina
Language:Chinese
Abstract: Objectives To study the expression features of C-type natriuretic peptide (CNP)/natriuretic peptide receptor-B (NPR-B) axis and two parallel elimination pathways, natriuretic peptide receptor-C (NPR-C) and neutral endopeptidase (NEP) in unilateral ureteral obstruction (UUO) rats. Methods CNP, NPR-B, NPR-C, NEP, Col-IV and type IV collagen (Col-IV) mRNA and proteins were determined by in situ hybridization, real-time PCR, immunohistochemistry and western blot in UUO rats at 24h, 72h, 1w, 2w, 3w, 1m, 2m and 3m. Results CNP expression tended to be higher immediately after ligation and de-clined along with the progression of disease, occurring predominantly in tubular epithelial cells. A high-level CNP may attribute to the elevated expression of NPR-B in the early phase of UUO. Conclusions NEP and NPR participate in the regulation of CNP expression in tubulointerstitial fibrosis. The gradual increased expression of NPR-C and NEP may cause the subsequent de-cline of CNP.