Efficiency of three methods for detecting Toxoplasma IgG antibody
- VernacularTitle:3种方法检测弓形虫IgG抗体的效能
- Author:
Yaxiao YANG
;
Yukun CHEN
;
Shijin WEI
;
Renhao SONG
- Publication Type:Journal Article
- Keywords:
Toxoplasma IgG antibody;
Gold marked method;
Indirect hemagglutination test(IHA);
Enzyme-linked immu-nosorbent assay(ELISA)
- From:
Chinese Journal of Schistosomiasis Control
2014;(1):109-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody. Methods To-tally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method,indirect hemagglutina-tion test(IHA),and enzyme-linked immunosorbent assay(ELISA),and the sensitivity,specificity and Youden index of these methods were compared. Results The detection sensitivities of gold marked method,IHA,and ELISA for Toxoplasma IgG anti-body were 85.5%,89.8%and 91.9%respectively(χ2=4.12,P>0.05);the specificities were 92.4%,96.6%and 97.5%respec-tively(χ2=4.06,P>0.05). The detection efficiency and Youden index of ELISA were 94.1%and 0.89 respectively,being high-er than those of IHA and gold marked method. Conclusion The sensitivity and specificity of the ELISA method for Toxoplasma IgG antibody are higher,and in addition,it can be automated. Therefore,it is suitable for large-scale Toxoplasma IgG antibody screening.
