Investigation of serum metabolic profiling in acute myocardium infarction
10.3760/cma.j.issn.1009-9158.2013.11.013
- VernacularTitle:急性心肌梗死患者血清代谢物谱的筛选研究
- Author:
Li ZHANG
;
Jie GAO
;
Fengmei ZHANG
;
Lei ZHANG
;
Hua KANG
;
Shuye LIU
- Publication Type:Journal Article
- Keywords:
Myocardial infarction;
Metabolomics;
Chromatography,high pressure liquid;
Mass spectrometry
- From:
Chinese Journal of Laboratory Medicine
2013;36(11):1022-1026
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the serum metabolic profiling of acute myocardium infarction (AMI) patients,establish a disease distinguishing model and to select characteristic metabolites with potential clinical diagnostic value.Methods All 42 AMI patients and contemporaneous 35 healthy physical examination adults from May 2011 to March 2012 at the Third Central Hospital of Tianjin.UPLC-LTQ Orbitrap XL MS platform filter characteristics of metabolites.SPSS 17.0 was used for statistical analysis.ROC curve was established to assess the clinical diagnostic value of selected characteristic metabolites.Results PCA (R2X =75.6％,Q2 =39.7％) model and OPLS-DA (R2Y =97.8％,Q2 =97.0％) model were established and 19 ions were identified.Glycerophospholipids decreased in AMI group and sphingophospholipids increased in AMI group.The areas under the curve of all identified metabolites were greater than 0.8.Conclusion Metabolites selected in metabolic profiling analysis show outstanding ability in distinguishing AMI from health people and can be used as potential diagnostic biomarkers and benefit in further clinical study as novel drug targets
