Typing of the PspA protein among Streptococcus pneumoniae strains isolated from children in Nan-jing Children′s Hospital

VernacularTitle:南京地区儿童肺炎链球菌临床分离株的PspA 蛋白分型
Author: Tianying ZHONG ; Tao ZHU ; Fei XU ; Xiameng MENG ; Zhongqi SHAO ; Yunsong CHANG ; Xuefeng YU
Publication Type:Journal Article
Keywords: Streptococcus pneumoniae; PspA; Vaccine; Coverage
From: Chinese Journal of Microbiology and Immunology 2013;(12):927-932
CountryChina
Language:Chinese
Abstract: Objective To investigate the coverage of a recombinant protein vaccine based on pneumococcal surface protein A (PspA) from both family 1 and family 2.Methods One hundred and fifty-nine Streptococcus pneumoniae strains, including 47 invasive strains, were isolated from children in Nanjing Children′s Hospital.Cell lysates were prepared and reacted with three antibodies recognizing PspA -RX1, PspA-3296 and PspA-5668 for PspA typing by ELISA .Results Among 47 invasive isolates of 9 different serotypes, 10.7%were PspA family 1 and 89.3%were PspA family 2.Among all of 159 clinical isolates, 10.1% were identified as PspA family 1, 88.0%were family 2, while 1.9%of strains could not be typed by ELISA and PCR assays .None of strains belonged to PspA family 3.Conclusion The recombinant pro-tein vaccine based on PspA from both family 1 and family 2 has a broad coverage among clinical isolates and is potentially protective against both invasive and non-invasive pneumococcal diseases .