Inhibitory effect of extracts from asparagus filicinus rhizome on proliferation of human osteosarcoma cells
10.3969/j.issn.1671-8348.2014.02.026
- VernacularTitle:羊齿天门冬根茎提取物对人骨肉瘤细胞增殖的抑制作用
- Author:
Jiaquan QU
;
Ying SHI
;
Wei JIA
;
Yongdong ZHANG
;
Fei ZHONG
- Publication Type:Journal Article
- Keywords:
liliaceae;
ethyl acetate fraction;
osteosarcoma;
cell proliferation;
cyclooxygenase 2
- From:
Chongqing Medicine
2014;(2):203-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of extracts from asparagus filicinus rhizome on prolifieration of human osteosarcoma Saos-2 cells and its molecular mechanism .Methods MTT assay was used to detect the cytotoxic activity and growth inhibition of three different extracts from asparagus filicinus rhizome against Saos-2 cells ;plate colony formation assay was per-formed to detect active fraction of asparagus filicinus rhizome on the anchorage dependent growth of Saos-2 cells ;the cell cycle alter-ation was determined by propidium iodide staining and flow cytometry analysis ;the alteration of protein expression level of COX-2 was determined by using Western blotting .Results Ethyl acetate fraction of asparagus filicinus rhizome (AF-A) exerted the potent cytotoxicity on Saos-2 cells(IC50 =26 .7 μg/mL);AF-A induced the inhibitory effect on the anchorage dependent growth of Saos-2 cells in a dose dependent manner(P<0 .05);Saos-2 cells treated by AF-A at the concentration of 30 .0 and 100 .0 μg/mL for 48 h induced the increase of percentages of S phage from (31 .8 ± 4 .8)% in the control group to (43 .7 ± 2 .5)% and(51 .9 ± 1 .9)% ,the difference showing statistical significance (P< 0 .05) .Western blotting showed that AF-A at different concentrations decreased COX-2 protein expression .Conclusion AF-A posseses the inhibitory effect on the proliferation and growth of human osteosarcoma cells in vitro ,and its mechanism might be associated with the induction of S phage arrest and the inhibition of COX-2 protein ex-pression level .
