Metabonomics research of acute renal graft rejection patients based on UPLC-qTOF-MS system
10.3760/cma.j.issn.1001-7097.2013.12.006
- VernacularTitle:基于UPLC-qTOF-MS的肾移植急性排斥反应患者血浆代谢组学分析
- Author:
Lei YE
;
Jingjing ZHANG
;
Hongyan LIU
;
Zenghua LIU
;
Wenwen LV
;
Jihong CHEN
- Publication Type:Journal Article
- Keywords:
Renal transplantation;
Graft rejection;
Diagnosis;
Metabonomics
- From:
Chinese Journal of Nephrology
2013;29(12):902-907
- CountryChina
- Language:Chinese
-
Abstract:
Objective Plasma phospholipid metabolism analysis was employed to investigate plasma metabolic profile of acute renal graft rejection in order to find potential disease biomarkers and reveal its pathophysiological changes.Methods Selected 33 patients which did renal transplant in our hospital during 2009 to 2010,named preoperative group.14 patients were diagnosed as acute graft rejection,and 19 patients were non-acute graft rejection.Then use ultra performance liquid chromatography (UPLC) coupled with quadrupole time of flight mass spectrometry (qTOF-MS) to establish the metabolic fingerprint in those patients.The date was preprocessed with software Markerlynx,and analyzed with partial least squares discriminant analysis (PLS-DA),and data from conventional laboratory techniques were used for assessing renal function.Results In the plasma of renal transplant patients,seventeen biomarkers were discovered:creatinine,four sphingomyelins (SM),nine phosphatidylcholines (PC),three lysophosphatidylcholine (LPC).While the creatinine was increased,the others were reduced obviously.Conclutions UPLC-qTOF-MS based metabonomics can analyze the plasma phospholipid metabolism in patients with renal transplantation,reveal the law of phospholipids metabolic changes in the process of acute rejection.The levels of plasma phosphatidylcholine's esterlysis and fatty acids' β-oxidation are increased.The immunosuppressive therapy can reduce the activity of phospholipase,inhibit the level of plasma phosphatidylcholine's esterlysis and fatty acids' β-oxidation,which restraines the development of acute rejection after renal transplantation.Therefore it may be a promising new technology in diagnosing acute renal graft rejection after renal transplantation.
