Expression of type IV collagen and laminin genes by skin fibroblasts in culture.
- Author:
Kyu Suk LEE
;
Jun Young SONG
- Publication Type:Original Article
- Keywords:
Type IV collagen;
Laminin;
Genes;
Fibroblasts
- MeSH:
Basement Membrane;
Collagen Type I;
Collagen Type IV*;
DNA, Complementary;
Fibroblasts*;
Gene Expression;
Humans;
Laminin*;
Peptides;
Procollagen;
RNA;
RNA, Messenger;
Skin*
- From:Korean Journal of Dermatology
1992;30(3):317-324
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Basement membrane zone gene expression by fibroblast cultures was examined by molecular hybridizations with human sequence specific cDNAs corresponding to type 1V procollagen and laminin subunit polypeptides. Northern transfer analysis with total RNA revealed the presence of specific mRNA transcripts for al (IV) and a2 (1V) chains of type 1V procollagen as well as Bl and B2 chains of laminin. Laminin A chain mRNAs were not detected using the same RNA preparations. The molecular size of al (1V) and a2 (1V) procollagen mRNA revealed 6.8kb and 6.7kb, respectively. The molecular size of Bl chain of laminin revealed 5.6kb, and B2 chain revealed 8.2 and 5.5kb polymorphic transcripts. In slotblot analysis using densitometer, steady-state levels of type IV procollagen and laminin mRNAs indicated that they were in relatively low abunclance, as compaired with type I procollagen mRNA. Quantitative levels of al (IV) and laminin Bl chaii mRNAs were more abundant than those of a2 (IV) and laminin B2 mRNAs. The mRNA ratio of al (IV)/a2 (lV) and laminin Bl/B2 were 1.9 and 1.5, respectively. These results demonstrate evidence for differential regulation of the expression of different basement membrane zone molecules during the formation of basement membrane.
