Acylated ghrelin and unacylated ghrelin inhibit INS-1 pancreas β-cell injury induced by sustained high glucose

VernacularTitle:乙酰化和非乙酰化胃促生长素对高糖诱导的大鼠INS-1胰岛β细胞损伤的影响
Author: Yong WANG ; Jian YANG ; Shuzhe ZHONG ; Jingang LIU
Publication Type:Journal Article
Keywords: Diabetes mellitus,experimental; Ghrelin; Pancreas β-cells; Endoplasmic reticulum stress
From: Chinese Journal of Endocrinology and Metabolism 2013;29(12):1052-1055
CountryChina
Language:Chinese
Abstract: Objective To examine whether ghrelin has beneficial effect on survival of pancreatic INS-1 beta cell.Methods Rat INS-1 cells were cultured separately in 5.6 mmol/L glucose (NG group),33.3 mmol/L glucose (HG group),33.3 mmol/L glucose plus 10 nmol/L acylated ghrelin (HG+AG group),and 33.3 mmol/L glucose plus 10 μmol/L unacylated ghrelin(HG+UG group).After being incubated for different hours,cell suvival rate was determined by MTT.Activity of caspase-3 was estimated by spectrophotometry,activity of GRP78,and cytochrome c was analyzed by confocal microscopy.Results Both acylated ghrelin and unacylated ghrelin inhibited the rise in activity of GRP78,caspase-3,and cytochrome c induced by sustained high glucose.Conclusions These findings indicate that ghrelin is able to inhibit endoplasmic reticulum stress and mitochondrial dysfunction of INS-1 β-cell caused by persistent high glucose,and the effect of ghrelin is not affected by acylation.