Icarrin increases bone morphogenetic protein 2 and Osterix expression in rat osteoblast
10.3760/cma.j.issn.1000-6699.2013.11.016
- VernacularTitle:淫羊藿甙促进体外培养成骨细胞骨成形蛋白-2及Osterix mRNA表达
- Author:
Shumei LI
;
Xingyi YANG
;
Yong LU
;
Xiuzhen ZHANG
- Publication Type:Journal Article
- Keywords:
Icarrin;
Osteoblasts;
Bone morphogenetic protein-2;
Osterix;
Osteoporosis
- From:
Chinese Journal of Endocrinology and Metabolism
2013;29(11):981-985
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of icarrin on proliferation and differentiation of rat osteoblasts cultured in vitro as well as the expressions of bone morphogenetic protein-2 (BMP-2) and Osterix in rat osteoblast.Methods Calvarial osteoblasts were obtained from newborn (＜ 24 h) SD rats by trypsin-collagenase digestion.The culture medium with different icarrin concentrations and the second generation osteoblasts were mixed.The mRNA expressions of BMP-2,Osterix,ALP,and Col Ⅰ were detected by semiquantative RT-PCR.Then the second generation osteoblasts were cultured in the medium containing icariin (10 ng/ml) with or without BMP-2 antibody.After48 h,RT-PCR was used to estimate the mRNA expression of Osterix.Results In comparison with the control group,the mRNA expressions of BMP-2,Osterix,ALP,Col Ⅰ were increased in a dose-dependent manner (P＜0.05) with a maximal effect at the concentration of 10 ng/ml.The mRNA expression of Osterix treated with the mixture of icarrin and BMP-2 antibody was significant decreased(P＜0.05),and compared with the group of icarrin,however,the difference between the BMP-2 group and the mixture group was not statistically significant (P ＞ 0.05).Conclusions Icarrin stimulates proliferation and differentiation of cultured osteoblast in vitro by increasing the mRNA expression of ALP and Col Ⅰ.Icarrin could stimulate Osterix gene expression by enchancing BMP-2 gene expression,finally,it could induce bone formation and prevent and/or treat osteoporosis.
