Correlation of telomerase reverse transcriptional gene expression with astrocyte activation
10.3760/cma.j.issn.1001-8050.2013.11.021
- VernacularTitle:端粒酶反转录酶基因表达与星形胶质细胞活化的相关性
- Author:
Mingkun YANG
;
Xu ZHANG
;
Chuan LIU
;
Jisheng WU
;
Deyi LI
;
Weibin SHENG
- Publication Type:Journal Article
- Keywords:
Spinal cord injuries;
Microglia;
Cicatrix;
Telomerase reverse transcriptase
- From:
Chinese Journal of Trauma
2013;29(11):1101-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between telomerase reverse transcriptase (TERT) gene expression and astrocyte activation.Methods Twenty neonatal 3-day-old male SD rats were used for culture of the astrocytes.The astrocytes were divided into Group A (activated,non-transfected astrocytes),Group B (activated,transfected astrocytes),Group C (unactivated astrocytes) and Group D (activated,empty plasmid transfected astrocytes) according to the random number table,with 5 rats per group.The cell proliferation rate in each group was detected by cell counting kit-8 (CCK-8) ;TERT expression by immunocytochemical method; expressions of TERT and glial fibrillary acidic protein (GFAP) genes by RT-PCR assay.Results Astrocytic proliferation ability in Group B lowered significantly as compared with that in Groups A,D and C (F =43.418,P ＜ 0.01).Expressions of TERT and GFAP mRNAs in Groups A and D were significantly higher than those in Group B and C,and no significant difference was found between Groups A and D.Besides,there was a linear correlation between mRNAs expressions of both genes in Groups A and D (r =0.701,0.704,P ＜ 0.01),while no significant linear correlation was observed in Groups B and C (r =0.260,P ＞ 0.05).Expressions of TERT and GFAP proteins in Groups A and D were markedly higher than those in Groups B and C and no significant difference was found between Groups A and D.Conclusion TERT genes are involved in the activation of astrocytes and exert effect on promoting the activation of astrocytes.
