Study on preparation of recombinant epidermal growth factor-loaded slow-release microspheres
10.3760/cma.j.issn.1008-1372.2013.11.023
- VernacularTitle:载转基因细胞的肌腱内固定用复合材料-rhEGF缓释微球的研究
- Author:
Jingqiang CAO
;
Liji LIU
;
Jian SHI
;
Chaoyue ZHANG
- Publication Type:Journal Article
- Keywords:
Epidermal growth factor;
Delayed-action preparations;
Polymers;
Polyglycolic acid;
Lactic acid;
Microspheres
- From:
Journal of Chinese Physician
2013;15(11):1522-1525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare poly (lactic-co-glycolic acid) (PLGA) microspheres that contain recombinant epidermal growth factor and then evaluate the effect of these sustained release in vitro.Methods Human serum albumin (HAS) microspheres were prepared with a double-emulsification solvent evaporation method.Experiment was designed to optimize the preparation condition of recombinant human epidermal growth factor (rhEGF)-loaded microspheres.Characters of optimal rhEGF-loaded microspheres were analyzed.In-vitro dissolution tests were performed on the microsperes.Results The speed of first emulsification,the concentration of PLGA,the concentration of polyvinyl alcohol (PVA),and the ratio of inner water to organic phase affected the particle size and the encapsulation ratio.The polymer's individual specificities especially density,molecular weight,polymerization,the diameter of microsphere,and the encapsulation efficiency were the crucial factors to influence the speed and time of drug release from microspheres in vitro.The optimal microspheres possessed a smooth and round appearance.It also showed good lente liberantes effect in vitro.Conclusions The optimal microspheres possessed a smooth and round stable appearance and showed good lente liberantes effect in vitro.This technique was simple and had a good reproducibility.
