Protective effects of ω-3 PUFA on the second liver injury in rats with traumatic brain injury and hemorrhagic shock
10.3760/cma.j.issn.1671-0282.2013.12.007
- VernacularTitle:ω-3PUFA对颅脑损伤伴休克大鼠继发性肝损伤的保护作用
- Author:
Huibin XU
;
Qing WANG
;
Huafen WANG
;
Qifeng LIU
;
Wengqing LIU
;
Yongqing WANG
- Publication Type:Journal Article
- Keywords:
Traumatic brain injury;
Hemorrhagic shock;
Liver injury;
ω-3 polyunsaturated fatty acid;
Oxygen free radicals
- From:
Chinese Journal of Emergency Medicine
2013;22(12):1338-1341
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of preconditioning with ω-3 polyunsaturated fatty acid (ω-3 PUFA) on the second liver injury in rats with traumatic brain injury and hemorrhagic shock (TBIS) and explore the underlying mechanism.Methods Total of 36 male Wistar rats were assigned randomly (random number) into 3 groups (n =12 in each):sham operation group (C),TBIS model group and PUFA pretreatment group.The arterial blood samples were taken for determination of serum levels of ALT,AST and TNF-α.The liver were removed for determination of levels of superoxide dismutase (SOD),malondialdehyde (MDA) and glutathione (GSH),and microscopic examination.Results Compared with group C,the serum levels of ALT,AST and TNF-α and the liver levels of MDA were significantly higher (P ＜0.01),but the liver levels of SOD and GSH in group TBIS and group PUFA were significantly lower (P ＜0.01).The serum levels of ALT,AST and TNF-α and the liver levels of MDA were significantly lower (P ＜ 0.01 or P ＜ 0.05),but the levels of SOD and GSH in liver tissues were significantly higher (P ＜ 0.01or P ＜ 0.05) in group PUFA than those in group TBIS.Histological examination revealed the injury of liver in TBIS group,and the rats in PUFA treated group showed alleviated severity of liver injury.Conclusions The supplementation of ω-3 PUFA can ameliorate acute liver injury in rats with TBIS,which may contribute to inhibition of oxygen free radicals and inflammatory cytokines expression.
