The involvement of outward potassium currents in methamphetamine-induced hippocampal neuron damage in fetal rat
10.3760/cma.j.issn.1671-0282.2013.11.007
- VernacularTitle:钾电流在甲基苯丙胺引起神经元损伤中的作用
- Author:
Lei JIANG
;
Jun WANG
;
Rong GAO
;
Hang XIAO
;
Jinsong ZHANG
- Publication Type:Journal Article
- Keywords:
Methamphetamine;
4-AP sensitive K+ channel;
TEA sensitive K+ channel;
Cell apoptosis;
Hippocampal neurons
- From:
Chinese Journal of Emergency Medicine
2013;22(11):1229-1234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of methamphetamine (Meth) on the outward K+ currents and elucidate the role of outward K+ channels in Meth induced hippocampal neuron damage.Methods Hippocampal neurons were harvest from 18-day-old embryonic rats and were divided into two groups:the control group and the Meth treated group.Both of 4-AP and TEA sensitive K+ currents were recorded after the treatment of Meth by performing the whole cell patch clamp.Furthermore,the MTT and TUNEL assays were performed to evaluate the effects of K+ channel on hippocampal neuron damage mediated by Meth.For statistical comparison,One-way ANOVA and LSD multiple comparison test or t-test was used.P-value ＜ 0.05 was considered to be statistically significant.Results The density of 4-AP sensitive K+ channel currents in Meth treated group [(120.1 ± 19.6) pA/pF,n =7] were significantly increased when compared with control group [(87.4 ± 12.5) pA/pF,n =10,P ＜0.01] and the increments of the currents induced by Meth was dose dependent.The MTT data showed that the cell viability was obviously decreased in Meth treated group (48.72 ± 4.38) ％ relative to the control group (100.07 ± 3.36) ％.Moreover,application of K+ channel antagonist,4-AP (61.39 ± 3.15)％,and the high K+ solution (78.25 ± 9.42) ％ substantially enhanced the cell viability.The TUNEL assay showed there were protective effects of 4-AP and the high K+ solution against neuron damage observed during cells exposed to Meth.Conclusions The increments of 4-AP sensitive K+ channel currents induced by Meth might be involved in hippocampal neuron damage.
