Effect of Tanyu-Tongzhi recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in rats
10.3760/cma.j.issn.1673-4246.2013.12.009
- VernacularTitle:痰瘀同治方对心肌缺血再灌注损伤大鼠心肌Rho/Rho激酶通路的影响
- Author:
Danli TANG
;
Lin TONG
;
Haifeng CUI
;
Yu SUI
;
Huamin ZHANG
- Publication Type:Journal Article
- Keywords:
Tanyu-Tongzhi recipe;
Myocardial ischemia-reperfusion injury;
Signal transduction pathway
- From:
International Journal of Traditional Chinese Medicine
2013;35(12):1082-1085
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Tanyu-Tongzhi (TYTZ)recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in high fat-fed rats.Methods 60 SD rats were divided into 5 groups randomly,a sham-operated group,a model group,a western medicine control group,a high-dose group and a low-dose group of TYTZ recipe.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.The downstream substrates of ROCK myosin light chain (MLC) phosphorylation expression of myocardial tissues in rats were detected by Western blotting method.The levels of RhoA,ROCK Ⅱ mRNA were determined with RT-PCR.Results Compared with the model group,the expression of p-MLC and RhoA,ROCK Ⅱ mRNA were lower in low-dose group of TYTZ,there were significant differences between the two groups(P=0.004、0.003、0.018、0.004,P＜0.01).Conclusion The TYTZ Recipe can protect myocardium from MI/RI.The mechanism of action was related to its inhibiting the protein expression of RhoA,ROCK Ⅱ and p-MLC,restraining the activation of Rho/Rho kinase signal transduction pathway.
