Analysis on lymphocytes subgroup and cytokines level of the long-term survival patients after OLT
10.3760/cma.j.issn.0254-1785.2013.11.008
- VernacularTitle:肝移植后长期生存受者外周血淋巴细胞亚群及细胞因子分析
- Author:
Junjun JIA
;
Li WANG
;
Jing ZHANG
;
Lei GENG
;
Lin ZHOU
;
Shusen ZHENG
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Lymphocyte;
Cytokines;
Immune tolerance
- From:
Chinese Journal of Organ Transplantation
2013;34(11):666-670
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characterization of the long-term survival patients through monitoring the lymphoeytes subgroup and cytokines level.Method The lymphocytes subgroups (T,B,and NK) and cytokines (IL-9,-17,-22) of patients and healthy groups were tested by using flow cytometry and ELISA.Results The levels of CD3+,CD4+,CD8+,CD8+ CD28+/CD8+ and IL-9 were gradually increased in the short-term group and long-term group as compared those in healthy group.The percentage of CD3 + cells and level of IL-9 were significantly lower in short-term group and long-term group than in healthy group (P＜0.05).The percentage of CD8+ cell was significantly lower in short-term group than in healthy group (P＜0.05).The ratio of CD8+ CD28+/CD8+ was significantly lower in short-term group than in longterm group and healthy group (P＜0.05).The percentage of B cells and NK cells,and IL-22 were gradually increased in the healthy group,shortterm group and long-term group.The percentage of B cells and NK cells was significamly higher in long-term group than in healthy group (P＜0.05).The level of IL-22 was significantly higher in longterm group than in short-term group and healthy group (P＜0.05).However,NKT lymnphocytes and IL-17 showed no statistically significant difference between long-term group and short-term group.Conclusion The ratio of T lymphocyte subgroups and the level of IL-9 were good biomarkers for evaluating the immune characterization of OLT patients; NK and B lymphocytes,and IL-22 may be associated with the long-term survival in patients after OLT.
