The role of Foxo1 and ubiquitin-proteasome system markers in muscle atrophy caused by chronic kidney disease

VernacularTitle:Foxo1及泛素溶酶体系统在慢性肾脏病致骨骼肌萎缩作用中的初步观察
Author: Jun YIN ; Juan HUANG ; Weijie YUAN ; Lijie GU ; Ling WANG ; Minghua SHANG
Publication Type:Journal Article
Keywords: Renal insufficiency,chronic; Rectus abdominis; Muscle atrophy; Foxo1; Ubiquitin-proteasome system
From: Chinese Journal of Internal Medicine 2014;53(1):31-34
CountryChina
Language:Chinese
Abstract: Objective To identity whether there is muscle atrophy phenomenon in end-stage kidney disease patients and to detect the level of transcription factor Foxo1 and the activity of ubiquitin-proteasome system.Methods Twenty-two patients in chronic kidney disease (CKD) stage 5 were selected and their mean muscle cross sectional area was measured.mRNA and protein levels of Foxo1,Atrogin-1,MuRF1 in rectus abdominis biopsies obtained from consecutive patients were detected.Control biopsies were obtained from 8 healthy subjects during elective surgery for abdominal wall hernias and 6 subjects during elective surgery for adenomyosis.Results Compared with the control group,cross sectional area of muscle fibers decreased and the transcription and protein levels of Foxo1,Atrogin-1,MuRF1 were upregulated in CKD group(P＜0.05).Protein level of p-Foxo1 decreased in CKD group(P＜0.05).Conclusion There exist muscle atrophy phenomenon in CKD patients,which may associate with the upregulation of Foxo1 and activation of ubiquitin-proteasome system.