Effects of limb ischemic preconditioning on intestinal injury during release of hepatic portal occlusion in rats
10.3760/cma.j.issn.0254-1416.2013.10.027
- VernacularTitle:肢体缺血预处理对大鼠肝门阻断开放时肠损伤的影响
- Author:
Xiaochun ZHENG
;
Wenshao TU
;
Fengyi HUANG
;
Ronggang LI
;
Jianghu CHEN
;
Yanping MENG
- Publication Type:Journal Article
- Keywords:
Ischemic preconditioning;
Hindlimb;
Liver;
Reperfusion injury;
Intestinal mucosa
- From:
Chinese Journal of Anesthesiology
2013;33(10):1252-1255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of limb ischemic preconditioning (LIP) on the intestinal injury during release of hepatic portal occlusion (RHPO) in rats.Methods Thirty-six male Sprague-Dawley rats,aged 6-7 weeks,weighing 200-250 g,were randomly divided into 3 groups using a random number table:sham operation group (group S,n =12),RHPO group (n =39) and LIP+ RHPO group (n =20).Hepatic ischemia/ reperfusion was produced by occluding portal vein and hepatic artery for 30 min followed by 6 h reperfusion according to Pringle's method.LIP was produced by tourniquet occlusion of the right femoral artery and vein for 10 min followed by 24 h reperfusion before hepatic ischemia.The animals were sacrificed at 6 h after hepatic portal occlusion was released and the mucous membrane of small intestine was obtained for microscopic examination and for determination of malondialdehyde (MDA) content,superoxide dismutase (SOD) activity,myeloperoxidase (MPO)activity,total antioxidant capacity (T-AOC) and intercellular adhesion molecule-1 (ICAM-1) mRNA and protein expression.The degree of damage to intestinal mucous membrane was scored according to Chiu.Results Compared with group S,Chiu's score and levels of MDA and MPO were significantly increased,SOD activity and TAOC were decreased,and ICAM-1 mRNA and protein expression was up-regulated in group RHPO (P ＜ 0.05 or 0.01).Compared with group RHPO,Chiu's score and levels of MDA and MPO were significantly decreased,SOD activity and T-AOC were increased,and ICAM-1 mRNA expression was down-regulated in group LIP + RHPO (P ＜ 0.05).Conclusion LIP can alleviate the intestinal injury during RHPO in rats by enhancing the antioxidant function and down-regulating the expression of ICAM-1.
