Expression of serum and glucocorticoid-regulated protein kinase 3 in breast cancer stem cells
10.3969/j.issn.2095-4344.2013.40.007
- VernacularTitle:血清和糖皮质激素调节蛋白激酶3在乳腺癌干细胞中的表达
- Author:
Hai XU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(40):7061-7067
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Serum and glucocorticoid-regulated protein kinase 3 may be related to tumor progression, and can prevent the apoptosis of breast cancer cells induced by interleukin-3 withdrawal. Serum and glucocorticoid regulated protein kinase 3 possibly participate in the regulation of intracellular Wnt signal pathway.
OBJECTIVE:To investigate the expression of serum and glucocorticoid-regulated protein kinase 3 andβ-catenin protein expression in breast cancer cells.
METHODS:Breast cancer stem cells were isolated, identified and subcultured from 71 breast cancer cases by dual-wave flow cytometry. The 30 normal breast tissues were used as the controls. SP immunohistochemistry analysis was used to detect the level of serum and glucocorticoid-regulated protein kinase 3 andβ-catenin protein expression in breast cancer stem cells and normal breast tissues.
RESULTS AND CONCLUSION:The expression of serum and glucocorticoid-regulated protein kinase 3 andβ-catenin in breast cancer stem cells were both higher than that of normal breast tissues (P<0.01). There had a positive correlation between serum and glucocorticoid-regulated protein kinase 3 andβ-catenin expression in breast cancer stem cells (r=0.318, P<0.05). Serum and glucocorticoid-regulated protein kinase 3 may play an important role in the occurrence and development of breast cancer by regulation of Wnt/β-catenin pathway.
