Influence of Sishen Pill on ICAM-1 mRNA and Protein Expression of Colonic Mucosa in Rats with Ulcerative Colitis
10.3969/j.issn.1005-5304.2013.10.009
- VernacularTitle:四神丸对溃疡性结肠炎大鼠结肠黏膜细胞间黏附分子-1基因和蛋白表达的影响
- Author:
Yan WANG
;
Wenjing TIAN
;
Xiangdong ZHU
;
Yanfei CAO
- Publication Type:Journal Article
- Keywords:
Sishen Pill;
ulcerative colitis;
ICAM-1;
protein expression;
gene expression;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(10):21-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Sishen Pill on ICAM-1 mRNA and protein expression of colonic mucosa in rats with ulcerative colitis (UC), and identify its mechanism. Methods Taolly 40 SPF Wistar rats were randomly divided into blank group, model group, Pill group and SASP group. Except the blank group, UC model was prepared with TNBS/ethanol enema. Pill group was given Sishen Pill 5 g/kg, and SASP group was given SASP 0.3 g/kg by gavage, blank group and model group was given the same volume physiological saline for three weeks. Morphological injury of colonic mucosa was observed and scored. ICAM-1 gene and protein expression were detected by RT-PCR and immunohistochemical method. Results Inflammation and ulceration were found on the colonic mucous membrane of rats in the model group. The expression of ICAM-1 gene and protein of colonic tissues of rats in the model group increased compared with that of the blank group (P<0.01). Compared with the model group, the expression of ICAM-1 in Pill group decreased (P<0.05, P<0.01). Conclusion Sishen Pill can decrease the expression of ICAM-1 mRNA and ICAM-1 protein, inhibit the infiltration of inflammation cells, prevent and reduce colon tissue damage, and play a vital role in the treatment of UC.
