Comparative analysis of immunological characteristics of patients with mild and severe influenza A (H1 N1)
10.3760/cma.j.issn.0254-5101.2013.09.007
- VernacularTitle:轻症和重症甲型 H1 N1流感患者免疫学特征比较分析
- Author:
Zongyi WANG
;
Airong HU
;
Suwen JIANG
;
Wangyi XUAN
;
Nanhong ZHENG
;
Guosheng GAO
;
Shixiong DING
;
Yaoren HU
;
Xiaoyue LIANG
- Publication Type:Journal Article
- Keywords:
Influenza A virus,H1N1 subtype;
Lymphocyte subsets;
Interferon-γ;
Interleukin-4
- From:Chinese Journal of Microbiology and Immunology
2013;(9):673-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To comparatively analyze the immunological characteristics of patients with mild and severe influenza A (H1N1), and to provide the evidence for condition monitoring and treatment . Methods 52 cases with mild influenza A ( H1N1), 152 cases with severe influenza A ( H1N1) and 26 healthy subjects from July 1, 2009 to December 31, 2009 were enrolled in the study.Lymphocyte subsets in peripheral blood were analyzed by flow cytometry and the serum concentrations of interferon -γ( IFN-γ) and interleukin-4 (IL-4) were detected by enzyme-linked immune-sorbent assay (ELISA).Results The total lymphocyte counts were decreased obviously in patients with severe influenza A ( H1N1) than in mild pa-tients and in healthy subjects (P<0.01).The T lymphocyte, NK cells, CD4+T lymphocyte and CD8+T lym-phocyte were also decreased obviously in severe patients than in mild patients (P<0.01).The B lymphocyte and CD4+/CD8+were also decreased in severe patients than in mild patients but had no significant statistical difference (P=0.11, 0.175).The serum IFN-γlevels in patients with mild and severe influenza A (H1N1) were lower than those in control group, especially in patients with severe influenza A (H1N1) (compared with control group and mild group , P<0.01).And the changes of serum IL-4 levels were the same with the former, but there were no statistically significant differences in three groups (P>0.05).Con-clusion Immune dysfunction in patients with influenza A (H1N1) infection is associated with the severity of disease, especially cellular immunity .Therefore, monitoring of the immune system is valuable for the diag-nosis of influenza A(H1N1) infection.
