Impacts of Kechuanning on Expression of Signal Transducer and Activator of Transcription 6 in CD4+, CD8+T Lymphocytes of Athma Rats Induced by Virus
10.3969/j.issn.1005-5304.2013.10.010
- VernacularTitle:咳喘宁对病毒诱发哮喘模型大鼠CD4+、CD8+T细胞内信号转导子和转录激活子6表达的影响
- Author:
Ying LI
;
Yinhe LUO
;
Mengqing WANG
;
Jie YAN
;
Ling LI
- Publication Type:Journal Article
- Keywords:
Kechuanning decoction;
bronchial athma;
CD4+T lymphocyte;
CD8+T lymphocyte;
STAT6;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(10):25-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impacts of Kechuanning on expression of signal transducer and activator of transcription 6 (STAT 6) in CD4+, CD8+T lymphocytes of athma rats induced by virus, and analyze the mechanism of Kechuanning in treating asthma induced by RSV. Methods The PBMC was isolated from the peripheral blood, CD4+T, CD8+T lymphocyte was separated by immunomagnetic beads. The purity and activity of CD4+T and CD8+T lymphocytes were measured by using flow cytometry, trypan-blue dye exclusion test. The protein expression of STAT6 was detected by fluorescence-activated cell sorting. Results The descending progress of the trial were supported by the purity and activity of CD4+T, CD8+T lymphocyte. CD4+T lymphocyte in the asthma model group were increased (P<0.01), however the increase of CD8+T lymphocyte were not significant (P>0.05). The expression of the protein of STAT6 in the treated group with Kechuanning decoction were decreased than that in the model group (P<0.01). Compared with Dexamethasone and Salbutamol group, there were no significant differences (P>0.05). Conclusion Kechuanning decoction could decrease the protein of STAT6 in CD4+, CD8+T lymphocyte, thereby it could regulate the disorder of Th1/Th2, thus prevent and treat the asthmatic attack induced by virus.
