Determination of Astragaloside IV in Yangxue Yishen Capsules by HPLC-ELSD

VernacularTitle:HPLC-ELSD测定养血益肾胶囊中黄芪甲苷的含量
Author: Juan PENG ; Han YAN ; Na GUO ; Yinglan NIE ; Bin FAN
Publication Type:Journal Article
Keywords: Yangxue Yishen Capsules; Astragaloside IV; TLC; HPLC-ELSD
From: Chinese Journal of Information on Traditional Chinese Medicine 2013;(7):52-54
CountryChina
Language:Chinese
Abstract: Objective To establish the quality standard for Yangxue Yishen Capsules. Methods Rhizoma Gastrodiae, Herba Epimedii and Radix et Rhizoma Salviae Miltiorrhizae in the formula were identified qualitatively by TLC. The content of Astragaloside IV was determined by HPLC-ELSD method. The Diamonsil C18(2) column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-water (33∶67) with 1.0 mL/min flow rate at 35 ℃. The temperature of drift tube for ELSD was 105 ℃, the air flow rate was 2.7 L/min. Results The TLC displayed clear spots on their right positions repeatedly. The linear range of Astragaloside IV was 0.397 0-5.955 0 μg (r=0.999 9). The average recovery was 102.0%, RSD was 1.5% (n=6). Conclusion The method is simple and quick. It can be used as quality control method of Yangxue Yishen Capsules.