Expression of LRP16 in human endometrial carcinoma tissues and its role on cell proliferation
10.3969/j.issn.1671-8348.2013.25.012
- VernacularTitle:LRP16基因在子宫内膜癌组织中的表达及其对细胞增殖的作用
- Author:
Yujiao ZHANG
;
Ling LIU
;
Chunyan WANG
;
Ping ZHAN
;
Zonghui WU
;
Wen HE
;
Dingyu WANG
- Publication Type:Journal Article
- Keywords:
endometrial carcinoma;
LRP16 gene;
HEC-1-B cells
- From:
Chongqing Medicine
2013;(25):2983-2985
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression change of LRP16 in endometrial cancer tissues and its influence on the pro-liferation of human endometrial carcinoma HEC-1-B cells .Methods HEC-1-B cells were transfected with LRP16 .RT-PCR was used to examine the expression of LRP16 in 26 normal endometrium specimens ,10 endometrial cancer specimens .RT-PCR was used for verifying the transfection success .WES-T was used to observe the proliferation change of HEC-1-B cells .Results The positive expression rate and level of LRP16 mRNA in the endometrial cancer tissues were 83 .33% and 0 .82 ± 0 .21 ,which were significantly higher than 30 .00% ,0 .47 ± 0 .18 in the normal endometrium tissues(P<0 .05) .The RT-PCR detection results revealed that the expression of LRP16 mRNA after transfection was significantly increased .HEC-1-B cells in the transfection group could continued to proliferate in vitro ,but the proliferation capacity was not increased .Conclusion The expression abnormality of LRP16 may be closely related to the occurrence and progress of endometrial cancer ,LRP16 gene may have potential value for the endometrial canc-er gene therapy .
