Observation on growth inhibition of sustained-release fluorouracil implantation in nude mice with human colorectal carcinoma
10.3969/j.issn.1671-8348.2013.26.002
- VernacularTitle:氟尿嘧啶缓释剂植入对人结直肠癌裸鼠移植瘤生长抑制的观察
- Author:
Hong LI
;
Liping YAN
;
Xiaoping SHI
- Publication Type:Journal Article
- Keywords:
colorectal neoplasms;
sustained-release fluorouracil;
caspase-3
- From:
Chongqing Medicine
2013;(26):3084-3086
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the growth inhibition effect of sustained-release fluorouracil implantation in nude mice with human colorectal carcinoma and to discuss its influence on caspase-3 expression .Methods The colorectal carcinoma subcutaneous transplanted model with LoVo cell in BALB/c nude mice was established and divided into 4 groups randomly :the tail intravenous injection of PBS (A group) ,the tail intravenous injection of 5-FU group (B group) ,the intratumor injection of 5-FU group (C group);and the implant of sustained-release fluorouracil group(D group) .After 15 d of medication ,the tumor weight and tumor volume were recorded ,the tumor inhibiting rates were calculated .The tumor tissues were observed by HE staining and detected the expression of caspase-3 by immunohistochemistry (IHC) .Results Sustained-release fluorouracil could suppress the transplantation tumor growth significantly ,the tumor weight and tumor volume in the D group had statistical differences compared with the A ,B and C groups(P<0 .05) ,which could up-regulated the expression of caspase-3 ,the differences between the D group with the A ,B and C groups (P<0 .05) .Conclusion Sustained-release fluorouracil can inhibit the grow th of colorectal carcinoma transplantation tumor more effectively than conventional fluorouracil and up-regulate the expression of caspase-3 ,which prompts that sustained-re-lease fluorouracil may play the anti-tumor role by inducing the tumor cell apoptosis via apoptosis pathway .Its definite tumor-inhibi-ting effect deserves to be promoted in clinical chemotherapy .
