Uric acid up-regulates the expression of prostanoid 2 in the glomerular mesangial cells through the p38MAPK pathway
10.3760/cma.j.issn.1001-7097.2013.06.011
- VernacularTitle:尿酸通过激活p38信号通路上调大鼠肾小球系膜细胞前列腺素E2的表达
- Author:
Junqiong PENG
;
Weijie YUAN
;
Wei ZHANG
;
Yi ZHOU
- Publication Type:Journal Article
- Keywords:
Uric acid;
Mesangial cells;
Dinoprostone;
p38 mitogen-activated protein kinases
- From:
Chinese Journal of Nephrology
2013;29(6):454-457
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the role and mechanism of p38 mitogerra activated protein kinase (p38MAPK) in up-regulation of prostanoid 2 induced by uric acid in rat glomerular mesangial cells (GMCs).Methods Rat glomerular mesangial cells were cultured in vitro,then stimulated with different concentrations of uric acid (50,100,300 μmol/L),or stimulated with different concentrations of uric acid (50,100,300 μmol/L) after pretreatment with p38MAPK specific inhibitor SC68376 (10 μmol/L) for 30 min.Activated p38MAPK was detected by Western blotting.The expression of prostanoid 2 was measured by ELISA.Cyclooxygenase-2 mRNA expression was determined by RT-PCR.Results Uric acid could activate p38MAPK and up-regulate the mRNA expressions of prostanoid 2 and cyclooxygenase-2 in rat glomerular mesangial cells (all P ＜ 0.05).SC68376 inhibited those effects of the above-described induced by uric acid.Conclusion Uric acid can promote the expression of prostanoid 2 in rat glomerular mesangial cells,whose mechanism may be related to the activation of p38MAPK and the promotion of cyclooxygenase-2 synthesis,and further up-regulation of prostanoid 2 expression.
