Expression and signiifcance of PLTP and MIF in mice with bronchopulmonary dysplasia
10.3969/j.issn.1000-3606.2013.09.019
- VernacularTitle:磷脂转运蛋白和巨噬细胞移动抑制因子在支气管肺发育不良小鼠血清中表达及意义
- Author:
Guifang LI
;
Baohai SHI
;
Guo YAO
;
Junli LIU
;
Chunhua QI
- Publication Type:Journal Article
- Keywords:
bronchopulmonary dysplasia;
macrophage migration inhibitory factor;
phospholipid transfer protein;
lung injury
- From:
Journal of Clinical Pediatrics
2013;(9):876-879
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and signiifcance of phospholipid transfer protein (PLTP) and macrophage migration inhibitory factor (MIF) in mice with bronchopulmonary dysplasia (BPD). Methods Ninety-six 4-day-old mice were randomly divided into oxygen group and air group. Mice in oxygen group were exposed to a FiO2 of 65%, and mice in air group were exposed to air. On day 7, 14, 21 and 28, blood and lung tissue samples from 12 randomly selected mice in each group were obtained. The serum levels of MIF and PLTP were measured by ELISA assay. The morphological changes of lung tissue were ob-served with HE staining. Results The mice in oxygen group showed thickened lung parenchyma and obvious pulmonary ifbrosis. The radioactive alveolar count was signiifcantly lower in oxygen group than that in air group (P<0.01). PLTP level in air group was increased gradually from day 7 to day 21, and began to decrease on day 28. PLTP level in oxygen group was increased from day 7 to day 14, and decreased on day 21 and day 28. MIF level in air group did not change during the experiment. MIF level in oxygen group was signiifcantly increased from day 7 to day 21, and began to decrease on day 28. Conclusions MIF and PLTP may be good biomarkers for the diagnosis of BPD.
