DNA methylation status of miR-126 and its host gene EGFL7 in CD4+T cells from patients with systemic lupus erythematosus

VernacularTitle:系统性红斑狼疮患者CD4+T细胞miR-126及宿主基因EGFL7 DNA甲基化状态分析
Author: Yunsheng LIANG ; Sha ZHAO ; Gongping LIANG ; Ming ZHAO ; Qianjin LU
Publication Type:Journal Article
Keywords: systemic lupus erythematosus; miR-126; EGFL7; host gene; DNA methylation
From: Journal of Central South University(Medical Sciences) 2013;38(8):793-797
CountryChina
Language:Chinese
Abstract: Objective:To explore the mechanisms by which DNA methylation regulates miR-126 and its host gene EGFL7 in CD4+T cells from patients with systemic lupus erythematosus (SLE).
Methods:We analyzed the expression and the DNA methylation status within promoter region of EGFL7 and miR-126 by real-time qPCR and bisulifte genomic sequencing analysis.
Results:miR-126 and EGFL7 mRNA expression was upregulated in CD4+T cells from SLE compared with that from healthy controls (P<0.01). EGFL7 mRNA level was positively correlated with miR-126 expression in CD4+T cells from SLE (r=0.538, P=0.015). The average methylation level of EGFL7 promoter in CD4+T cells from SLE was lower than that from healthy controls (P<0.05).
Conclusion:hTe upregulation of miR-126 and its host gene EGFL7 expression in CD4+T cells from SLE is associated with the hypomethylation of the EGFL7 promoter.