miR-126 inhibits colon cancer proliferation and invasion through targeting IRS1, SLC7A5 and TOM1 gene
10.3969/j.issn.1672-7347.2013.08.009
- VernacularTitle:miR-126靶向调控IRS1，SLC7A5及TOM1基因抑制结肠癌的增殖及侵袭转移
- Author:
Nan LI
;
Xiayu LI
;
Shuo HUANG
;
Shourong SHEN
;
Xiaoyan WANG
- Publication Type:Journal Article
- Keywords:
miR-126;
colon cancer;
lentivirus transfection;
target gene;
metastasis
- From:
Journal of Central South University(Medical Sciences)
2013;38(8):809-817
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression pattern and function of miR-126 in human colon cancer and the underlying mechanisms.
Methods:hTe expression pattern of miR-126 in high-density human colon cancer tissue microarray was analyzed by in situ hybridization. Further more, the biological function of miR-126 in colon cancer in vitro was investigated by establishing a stable miR-126 over-expression cell lines.
Result:hTe expression of miR-126 was lower in the tumor tissue, especially in metastasis tissue. hTe down-regulation of miR-126 was more obvious in the patients who displayed bad prognosis (P=0.025). Over-expression of miR-126 in colon cancer cell was able to inhibit cell proliferation, promote cell apoptosis and reduce the invasive ability. MiR-126 significantly enhanced the sensitivity of the colon cancer cell to chemotherapeutic drug. It has been shown that IRS1, SLC75A and TOM1 were the potential target genes of miR-126 in colon cancer.
Conclusion:MiR-126 was able to inhibit the development of colon cancer and its level was closely related with the prognosis of patients with colon cancer. The potential target genes for miR-126 might include IRS1, SLC7A5 and TOM1. Therefore, miR-126 might be a therapeutic target for colon cancer diagnosis and treatment.
