Correlation of WIF-1 expression and clinicopathologic significance in breast cancer with aberrant DNA methylation
10.3969/j.issn.1000-8179.20130481
- VernacularTitle:乳腺癌WIF-1基因表达及其临床病理特征与该基因启动子区域甲基化的关联*
- Author:
Jinlian SONG
;
Zhongliang MA
;
Lin HOU
;
Chunhong MA
;
Shengnan WANG
;
Li WU
- Publication Type:Journal Article
- Keywords:
WIF-1;
gene expression;
breast cancer;
methylation;
TNM stage
- From:
Chinese Journal of Clinical Oncology
2013;(18):1098-1101
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mRNA expression of the WIF-1 gene and the methylation of its promoter in breast can-cer, and to determine the correlation between the epigenetic aberrant WIF-1 DNA methylation and the clinicopathological significance of WIF-1 in breast cancer . Methods:RT-PCR and sensitive methylation-specific-PCR (MSP) were used to detect WIF-1 mRNA ex-pression and the methylation of the WIF-1 promoter in 30 breast cancer samples as well as in tumor-adjacent tissue samples and 9 be-nign breast tissues. Results:The WIF-1 mRNA expression in 30 breast cancer samples significantly decreased compared with those of the other two groups. In addition, WIF-1 methylation was more frequent in breast-tumor tissues compared with those in tumor-free tis-sues. Meanwhile, WIF-1 mRNA expression in breast cancer tissues involved the abnormal methylation of its promoter. Clinicopatholog-ical correlation analysis showed that the abnormal methylation of the WIF-1 gene promoter was not associated with age, TNM stage, histotype, or lymph node metastasis. Conclusion:WIF-1 mRNA expression loss due to abnormal methylation may be a crucial factor in breast cancer development and can thus be used in the prognosis and progression of the disease.
